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Cultivation Library

DIY Mycology: Resourceful Tips for Liquid Culture & Lab Work

2025-02-23  /  2 min  /  173 words

Like, uh, the honey, 1 tbspoon of Honey to 500 M of water, and then pressure sterilized in a little pressure cooker for 20 minutes, and then take a liquid culture and, uh, after the flame, uh, after you flame sterilize the tip and needle, inject it right into your injection Port. You only need a drop, and then from there you can grow out more of your own liquid culture. With that liquid culture, you can use it to inoculate your own grain based substrates and make your own spawn, so that's what I'd recommend for a home cultivator. Can also buy prepoured Petri dishes from other people or other Growers and, uh, just do the transfer yourself. If you can't get access to this parap film, I've seen other people online will use, like, a Saran Wrap.

Um, I have never had to use that, but that's just an idea if you, if you're ever in that situation. Everybody loves shiitake mushrooms. It's definitely one that everybody's familiar with, so oh.

Written protocol

Southwest Mushrooms Genetic Library

The strains themselves, the same cultures used in this footage.

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